Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1994-01-27
1994-11-08
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435 13, 435 4, 436 66, 436904, C12Q 128, C12Q 126, C12Q 156, G01N 3372
Patent
active
053626338
ABSTRACT:
A method of determining the presence or concentration of a peroxidatively active substance, such as hemoglobin, in a test sample is disclosed. The method comprises contacting the test sample with an indicator reagent composition which interacts with the peroxidatively active substance to produce a measurable or detecable color change. The indicator reagent composition comprises a redox indicator dye, a hydroperoxide, a ferric ion and a phosphorous compound having the formula ##STR1## wherein R.sub.1 or R.sub.2 are certain substituted or an unsubstituted aromatic moieties or certain polyhydric compounds, and wherein X is O, S or NH. The assay can be performed in wet phase or the composition can be incorporated into a carrier matrix. The method provides a more accurate and trustworthy assay of a test sample for a peroxidatively active substance and is especially useful in the assay of urine for occult blood.
REFERENCES:
patent: 4288541 (1981-09-01), Mager et al.
patent: 4310626 (1982-01-01), Bunkhardt et al.
patent: 4615982 (1986-10-01), Lawrence
Coe Roger N.
Miles Inc.
Robinson Douglas W.
Weber Jon
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