Methods and compositions for enzyme complementation assays using

Details Methods and compositions for enzyme complementation assays using Methods and compositions for enzyme complementation assays using

1993-05-25

1994-11-08

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, C12Q 134, G01N 33535

Patent

active

053626257

ABSTRACT:
The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the .beta.-galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypeptide synthesis techniques, which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase, is described along with improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample.

REFERENCES:
patent: 4378428 (1983-03-01), Farina et al.
patent: 4708929 (1987-11-01), Henderson
Ullmann, A. and Perrin, D. "Complementation in .beta.-galactosidase," in The Lactose Operon (ed. J. R. Beckwith and D. Zipser.), pp. 143-172, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1970).
Celada, F., Ullmann, A. and Monod, J. "An Immunological Study of Complementary Fragments of .beta.-Galactosidase" Biochemistry, vol. 13 (27), pp. 5543-5547 (1974).
Welphy, et al., J. Biol. Chem., 256:6804 (1981).
Langley, et al., Biochemistry, 15:4866 (1976).
Langley, et al., Proc. Natl. Acad. Sci. USA, 72(4):1254.

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