Process for separating and measuring glycoprotein

Details Process for separating and measuring glycoprotein Process for separating and measuring glycoprotein

1994-11-14

1997-01-07

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 79, 435 18, 435973, 436827, 530412, 530413, G01N 3353

Patent

active

055915892

ABSTRACT:
The degree of a structure change of the sugar chain of a glycoprotein caused by a disease can be measured with high precision rapidly by separating and measuring two or more forms of glycoproteins which are different in sugar chain structure but have substantially the same protein structure, by using a combination of a lectin capable of recognizing the specific sugar chain structure of at least one of these glycoprotein analytes to be measured, and a first antibody which has a property of binding to all the glycoprotein analytes but is kept from binding to glycoprotein analyte(s) having the lectin attached thereto; and separating and measuring glycoprotein analyte(s) having the first antibody attached thereto and glycoprotein analyte(s) having no first antibody attached thereto, by utilizing differences in properties between the former glycoprotein analyte(s) and the latter glycoprotein analyte(s).

REFERENCES:
patent: 4371515 (1983-02-01), Chu
patent: 4389392 (1983-06-01), Adachi
Chemical Abst., vol. 118, No. 21, 1993 May 24, Columbus, OH; Katoh et al., "Analytical method for sugar chain structures involving lectins . . .", p. 470; 208 749p; J. Biochem (Tokyo) 1993, 113(1), 118-22.
Chemical Abst., vol. 115, No. 19, 1991 Nov. 11, Columbus, OH; Taniguchi et al., "Quantitative determination of sugar chains in glycoproteins . . .", p. 482; 202 251m; Seibutsu Butsuri Kagaku, 1991, 35(3), 199-204.
Chemical Abst., vol. 110, No. 19, 1991 May 8, Columbus, OH; Taketa et al., "Application of lectin affinity electro-phoresis . . .", p. 482; 202 251m; Rinsho Byori, Rinji Zokan 1988; (79), 140-8.
Aoyagi et al, Biochemica et Biophysica Acta, 830, (1985), pp. 217-223.

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