Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-05-20
1997-01-07
Ketter, James S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351723, 4353201, 536 232, C12Q 168, C12N 1564, C12N 1577, C12N 1552
Patent
active
055915779
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
This invention relates to a DNA fragment containing a mobile genetic element originated from a strain belonging to the genus Brevibacterium and a process for obtaining said DNA fragment, to a plasmid vector constructed using said DNA fragment and a process for transferring a gene into chromosome making use of said vector and to a strain of Brevibacterium obtained by said process, where said mobile genetic element is an essential factor for the chromosome engineering-aided improvement of Brevibacterium strains which are important in the amino acid industry.
BACKGROUND ART
Intensive studies have been carried out in the prior art on the breeding and improvement of strains of the genus Brevibacterium in order to achieve efficient production of amino acids. As a means for use in the breeding, a large number of genetic engineering techniques have been reported. Gene manipulation techniques for use in the breeding of strains belonging to the genus Brevibacterium have been developed making use of plasmid and phage systems, such as establishment of protoplast transformation methods (Katsumata, R., Ozaki, A., Oka, T. and Furuya, A.: J. Bacteriol. 159 (1984) 306-311, Santamaria, R. I., Gil, J. A. and Martin, J. F.: J. Bacteriol. 161 (1985) 463-467), development of various vectors (Miwa, K., Matsui, K. , Terabe, M., Nakamori, S., Sano, K. and Momose, H.: Agric. Biol. Chem. 48 (1984) 2901-2903, Katsumata, R., Ozaki, A., Oka, T. and Furuya, A.: J. Bacteriol. 159 (1984) 306-311, Santamaria, R., Gil, J. A., Mesas, J. M. and Martin, J. F.: J. Gen. Microbiol. 130 (1984) 2237-2246, Patek, M., Nesvera, J. and Hochmannova, J.: Appl. Microbiol. Biotechnol., 31 (1989) 65-69, Yeh, P., Oreglia, J., Prevotos, F. and Scicard, A. M.: Gene 47 (1986) 301-306), development of gene expression controlling method (Tsuchiya, M. and Morinaga, Y.: Bio/Technology 6 (1988) 428-430) and development of cosmids (Miwa, K. , Matsui, K. , Terabe, M., Ito, K., Ishida, M., Takagi, H., Nakamori, S. and Sano, K.: Gene 39 (1985) 281-286). Also reported are cloning of genes making use of such systems (Melumbres, M., Mateos, L. M. , Guerrero, C. and Martin, J. F.: Nucleic Acids Res., 16 (1988) 9859, Mateos, L. M., Del, R. G., Aguilar, A. and Martin, J. F.: Nucleic Acids Res., 15 (1987) 10598, Matsui, K., Miwa, K. and Sano, K.: Agric. Biol . Chem. 52 (1988) 525-531, Mateos, L. M., Del, R. G., Auilar, A. and Martin, J. F.: Nucleic Acids Res., 15 (1987) 3922, Matsui, K., Sano, K. and Ohtsubo, E.: Nucleic Acids Res., 14 (1986) 10113-10114, Peoples, O. P., Liebl, W., Bodis, M., Maeng, P. J., Follettie, M. T., Archer, J. A. and Shinskey, A. J.: Mol. Microbiol., 2 (1988) 63-72, Eikmanns, B. J., Follettie, M. T., Griot, M. U., Martin, U. and Shinskey, A. J.: Mol. Gen. Genet., 218 (1989) 330-339, O'Regan, M., Thierbach, G., Bachmann, B., VGilleval Lepage, P., Viret, J. F. and Lemoine, Y.: Gene 77 (1989) 237-251, Follettie, M. T. and Shinskey, A. J.: J. Bacteriol., 167 (1986) 695-702) and yield improvement of various amino acids (Sano, K., Ito, K., Miwa, K. and Nakamori, S.: Agric. Biol. Chem., 51 (1987) 597-599).
However, there are no reports on mobile genetic elements originated from strains belonging to the genus Brevibacterium.
Mobile genetic element is a DNA fragment which can move on the chromosome and its existence is known in a large variety of organisms ranging from procaryotic to eucaryotic organisms, especially in detail in corn, Drosophila, yeast and the like as eucaryotic organisms and Escherichia coli and the like as procaryotic organisms (Mobile DNA, American Society for Microbiology, Washington D.C. (1989)). Also, Japanese Patent Application Laying Open (Kokai) No. 63-24889 discloses a mobile genetic element originated from Corynebacterium diphtheriae and European Patent Application 0,445,385 A discloses a transposon originated from a bacterium belonging to the genus Corynebacterium.
Mobile genetic elements of bacterial origins are divided into insertion sequence and transposon. Insertion sequence is a DNA fragment having a size of
REFERENCES:
Tsuchiya et al., Bio/Technology, vol. 6, 1988, pp. 428-430.
Miwa Kiyoshi
Moriya Mika
Tsuchiya Makoto
Ajinomoto Co. Inc.
Ketter James S.
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