Protease resistant PDGF and methods of use

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.

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530350, C12N15/18

Patent

active

059051426

ABSTRACT:
PDGF B-chain polypeptides characterized by the substitution or deletion of a lysine or arginine residue at position 27, 28, 32, 79, 80 or 81 of native B-chain are disclosed. Also disclosed are PDGF-like proteins containing the B-chain polypeptides. The polypeptides and proteins may be produced through the use of cultured cells transfected or transformed to express DNA molecules that encode the polypeptides. The PDGF-like proteins are useful as components of cell culture media and for accelerating the healing of wounds.

REFERENCES:
patent: 4845075 (1989-07-01), Murray et al.
patent: 5045633 (1991-09-01), Murray et al.
patent: 5512545 (1996-04-01), Brown et al.
Johnsson et al., "The c-sis gene encodes a precursor of the B chain of platelet-derived growth factor," EMBO J. 3(5):921-928, 1984.
Betsholtz, et al., "cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines," Nature 320:695-699, 1986.

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