Method for performing site-specific affinity fractionation for u

Details Method for performing site-specific affinity fractionation for u Method for performing site-specific affinity fractionation for u

1996-12-17

1999-05-18

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 9121, 427 211, 436 94, 436800, 436807, 436809, 436527, 436528, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q1/68

Patent

active

059050241

ABSTRACT:
A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.

REFERENCES:
patent: 5503980 (1996-04-01), Cantor
patent: 5525464 (1996-06-01), Drmanac et al.
patent: 5552270 (1996-09-01), Kharpko et al.

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