Hybridoma antibody (FH6) defining a human cancer-associated difu

Details Hybridoma antibody (FH6) defining a human cancer-associated difu Hybridoma antibody (FH6) defining a human cancer-associated difu

1985-08-07

1990-02-27

Tarcza, John Edward

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435948, 435 7021, 436548, 935104, 935110, 530387, C12N 500, C12P 2100, A61K 39395, G01N 3354

Patent

active

049045962

ABSTRACT:
A hybridoma cell line (ATCC No. HB 8873) secreting a monoclonal IgM antibody (FH6) directed to a fucoganglioside, 6B, which accumulates in human colonic adenocarcinoma but is absent in normal colonic mucosa. The structure of the 6B ganglioside to which the antibody FH6 is directed is as follows: ##STR1## The hybridoma secreting the antibody FH6 was selected by reactivity of the FH6 antibody with the 6B ganglioside (VI.sup.3 NeuAcV.sup.3 III.sup.3 Fuc.sub.2 nLc.sub.6) and lack of reactivity with other glycolipids, including glycolipids having closely related structures, such as sialosyllactoneotetraosylceramide (IV.sup.3 NeuAcnLc.sub.4), sialosyllactofucopentaosy(III)ceramide (IV.sup.3 NeuAcIII.sup.3 FucnLc.sub.4), sialosyllactofucopentaosy(II)ceramide (sialosyl-Le.sup.a glycolipid; IV.sup.3 NeuAcIII.sup.4 FucLc.sub.4), and 6C fucoganglioside (sialosyl 2.fwdarw.6 fucoganglioside; VI.sup.6 NeuAcIII.sup.3 FucnLc.sub.6). The antibody FH6 is highly reactive with a large variety of human cancer cells, including colonic, lung, and breast cancer, but does not react with most normal adult cels (except, notably, granulocytes). The antibody FH6 is of practical value in diagnostic tests and in monitoring and implementing various cancer treatments.

REFERENCES:
patent: 4444744 (1984-04-01), Goldenberg
patent: 4471057 (1984-09-01), Koprowski et al.
Fukushi et al., Cancer Research 45, pp. 3711-3777 (1985).
Magnani et al., Journal of Biological Chemistry 257 (23) pp. 14365-14369 (1982).
Vitetta et al., Science 219, pp. 644-650 (1983).
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Cancer Research 44:5279-5285, 1984.
Cancer Research 45:435-437, 1985.

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