Carbomycin resistance-conferring gene, designated cara, for use

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435886, 43525235, 4351721, 4352535, 435320, 536 27, 935 14, 935 19, 935 27, 935 60, C12R 1465, C07H 1512, C12N 1500, C12N 120, C12N 100, C12P 2100

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049045911

ABSTRACT:
The carA gene is a novel carbomycin resistance-conferring gene isolated from Streptomyces thermotolerans and used to construct a number of cloning vectors for use in Streptomyces and related organisms. One such cloning vector, plasmid pOJ158, can be obtained in S. griseofuscus C581 under the accession number NRRL 18089. S. lividans and S. griseofuscus are the preferred hosts when the carA gene is used to select carbomycin-resistant Streptomyces transformants.

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