Purified Mullerian Inhibiting Substance and method of purificati

Details Purified Mullerian Inhibiting Substance and method of purificati Purified Mullerian Inhibiting Substance and method of purificati

1981-07-29

1983-09-13

Rosen, Sam

Drug, bio-affecting and body treating compositions

Fermentate of unknown chemical structure

Having a known elemental analysis

A61K 3548

Patent

active

044041888

ABSTRACT:
A process for purifying Mullerian Inhibiting Substance (MIS) from testicular tissue which comprises incubating testicular tissue in a culture media in the presence of a protease inhibitor in order to extract MIS from the tissue, chromatographing the extract on an anionic exchange resin followed by chromatographing the biologically active product obtained therefrom on a cationic exchange resin; the biologically active material from the cationic exchange resin is chromatographed by lectin affinity chromatography on wheat germ lectin and then either chromatographed by lectin affinity chromatography on concanavalin A or by triazinyl dye affinity chromatography on an appropriate triazinyl dye; MIS is obtained in 8,000-fold and 15,000-fold purification over original testicular tissue.

REFERENCES:
Swann et al., Chem. Abst., vol. 90, (1979), p. 199833k.
Josso et al., Chem. Abst., vol. 92, (1980), p. 210550e.
Swann et al., Developmental Biology 69, 73-84, (1979).
Donahoe et al., Pediatric Andrology, 37-46, 1981, Martinus Nijoff Publishers, The Hague/Boston/London.
Donahoe et al., Science, vol. 205, 913-915, (1979).
Budzik et al., Cell, vol. 21, 909-915, (Oct. 1980).
Mudjett-Hunter et al., 1981 FASEB Abstract Form Entitled "Monoclonal Antibody to Mullerian Inhibiting Substance", an Abstract from a Meeting Held in Apr. of 1981.
Donahoe et al., Biology of Reproduction 16:238-243, (1977).
Picard et al., Biomedicine 1976, 25:147-150.
Josso, Journal of Clinical Endocrinology, 34-265-270, (1972).
Josso et al., Biology of Reproduction, 13:163-167, (1975).
Josso et al., "In Vitro Synthesis of Mullerian Inhibiting Hormone by Seminiferous Tubules Isolated from the Calf Fetal Testis", (1973), pp. 829-834.

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