Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1995-07-12
1998-06-16
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435182, 435382, 435395, C12N 1110, C12N 1104, C12N 500
Patent
active
057669074
ABSTRACT:
A method is provided for immobilization of whole microbial cells containing enzymes in Ca-alginate capsules. Whole microbial cells are mixed with a CaCl.sub.2 solution, a small amount of xanthan gum is added, the resultant mixture is added drop-wise while stirring to a Na-alginate solution containing a small amount of surfactant which is preferably polyoxyethylenesorbitan monolaurate to obtain the capsules containing the cells. The capsules may be washed with distilled water, hardened by in a CaCl.sub.2 solution and incubated in a growth medium. The microbial cells are bacteria or fungi cells and may be recombinant cells.
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Chang Ho-Nam
Park Joong-Kon
Seo Jin-Ho
Seong Gi-Hun
Yoo Ik-Keun
Korea Advanced Institute of Science & Technology
Naff David M.
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