Methods and compositions for high protein production from non-na

Details Methods and compositions for high protein production from non-na Methods and compositions for high protein production from non-na

1995-06-05

1997-05-20

Adams, Donald E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351722, 4353201, 435 7021, 4352523, 5303873, 530867, 536 2353, 536 241, 536 2372, C12N 516, C12N 1509, C12N 1513, C12N 1533

Patent

active

056311583

ABSTRACT:
Disclosed herein are improved methods and compositions for achieving enhanced protein production expressed from non-native gene constructs, including single chain sFv and derivative sequences. The methods and compositions are particularly useful for creating stably transfected, contitutively expressing immortalized mammalian cell lines that exhibit high recombinant protein productivity while maintaining a low copy number per cell of the non-native recombinant DNA sequence encoding the protein of interest.

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