Method and apparatus for improved analytical fluorescent spectro

Details Method and apparatus for improved analytical fluorescent spectro Method and apparatus for improved analytical fluorescent spectro

1975-06-30

1977-11-15

Willis, Davis L.

Radiant energy

Luminophor irradiation

With ultraviolet source

G01N 2138

Patent

active

040587320

ABSTRACT:
A method is disclosed whereby the molecules of a chemical substance to be measured, referred to as target molecules, are combined with fluorescent molecules having a relatively long fluorescent decay lifetime. These tagged target molecules are excited with an intense pulse of ultraviolet or visible radiation, and a detection system is gated on only after background fluorescence has substantially decayed, but while the fluorescent tag attached to the target molecules is still actively decaying. The amount of fluorescent tag emission which is measured is indicative of the target molecule content of the sample. An apparatus for improved fluorescent spectrofluorometry includes a pulsed excitation source and a gating means connected to a detection system for gating the detection system on after tagged target molecules have been excited and competing background fluorescence has substantially decayed, but before tagged target molecules have substantially decayed.

REFERENCES:
patent: 3842264 (1974-10-01), Arnold et al.
patent: 3886363 (1975-05-01), Ohnishi et al.
patent: 4006360 (1977-02-01), Mueller
"Gated Nanosecond Time Resolved Emission Spectroscopy Separation of Mixed Emissions from Carbonyl Compounds", R. E. Brown et al., Analytical Chemistry, vol. 46, p. 1690 (1974).

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