Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1984-10-09
1989-07-18
Rosen, Sam
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 68, 435180, 436512, C12P 2106
Patent
active
048493520
ABSTRACT:
F(ab) fragments are isolated from an antibody containing source by contacting the antibody containing source with a papain-polyacrylamide matrix to produce F(ab) and F(c) fragments which are then passed through an antigen-polyacrylamide gel capable of attracting the F(ab) fragments. F(ab).sub.2 fragments are obtained by contacting the antibody containing source with a pepsin-polyacrylamide matrix to produce F(ab).sub.2 and F(c) fragments which are then passed through an antigen-polyacrylamide gel capable of attracting the F(ab).sub.2 fragments. IgG antibodies are obtained by passing an antibody containing source through an antigen-polyacrylamide gel. These processes can be used to purifiy a wide variety of antibodies which can be used as therapeutic agents and as diagnostic agents. Antivenins produced by these processes have substantially reduced foreign protein levels and hence are less likely to produce immunogenic reactions. Bulk, unprocessed antibody sources may be utilized and, for reasons of process simplification, are preferred.
REFERENCES:
patent: 4012502 (1977-03-01), Philpot
Bernfeld--Science, 142:678-679, (1963).
Martinez-Hernandez et al.--Journal of Histochemistry and Cytochemistry, 23(2):146-148, (1975).
Sullivan et al.--Proc. West. Pharmacol. Soc., 25:185-192, (1982).
Otten--American Journal of Emergency Medicine, 1:83-93, (1983).
Smith et al.--Clin. Exp. Immunol., 36:384-396, (1978).
Carrel et al.--Nature, 221:385-386, (1969).
Nisonoff--A. Methods Medical Res., 101:134-141, (1964).
Kolb et al.--Chem. Abst., vol. 97, (1982), p. 90190s.
Coulter et al.--Chem. Abst., vol. 99, (1983), p. 3883v.
Russell Findlay E.
Sullivan John B.
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