Method of speciated isotope dilution mass spectrometry

Radiant energy – Ionic separation or analysis – Methods

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250282, B01D 5944, H01J 4900

Patent

active

054142598

ABSTRACT:
The method of determining the concentration of a specie in a sample includes providing at least one predetermined, enriched isotope in the same speciated form as the species to be measured, spiking the sample containing the species to be measured, equilibrating the spiked species with the species to be measured, separating the species from the sample and subsequently determining the concentration of the species to be measured by employing isotopic element specie ratios. In one embodiment, a single speciated isotope spike is employed and, in others, two or more such spikes may be employed. In a preferred embodiment, time resolution chromatography is used to effect separation of these species from the sample and mass spectrometer is employed in determining isotopic elemental ratios. It is also preferred that a method be employed to determine if there has been conversion from one species to another. In another embodiment, spiking of the several different isotopically enriched analogs of the same specie are added at various steps in the sampling procedure and the stability and integrity of the specie with respect to these processes is evaluated by mass spectrometric measurements of the various isotopic ratios. Chemical processes, extraction methods, dissolution procedures and storage procedures are evaluated. In another embodiment, speciated isotope dilution is used to determine the effect on species of various sample preparation methods and portions of sample preparation techniques. Extraction and separation procedures employ the technique to provide definitive evidence of accurate specie manipulation and provide for performance based measurement.

REFERENCES:
patent: 3786249 (1974-01-01), Anbar et al.
patent: 4022876 (1977-05-01), Anbar
patent: 5352893 (1994-10-01), Freedman

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