Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-05-01
1997-03-18
LeGuyader, John L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536232, 536245, 435 6, 435 911, 435 9131, 4351721, C12N 1511, C12Q 168
Patent
active
056124694
ABSTRACT:
Methods are disclosed for increasing ribozyme catalytic activity without reducing specificity, which methods comprise contacting an RNA molecule with a ribozyme and a facilitator oligonucleotide.
The present invention further provides compositions comprising a ribozyme and an effective amount of a facilitator oligonucleotide.
REFERENCES:
Uhlmann et al. Chemical Reviews 90:544-575 (1990).
Sarven et al. Science 247:1222 (1990).
Rossi et al. J. Cell. Biochem. Suppl. 14A, D428 (1990).
Muesing et al Nature 313:450 (1985).
Haseloff et al (1988) Nature 334, 585-591.
Hortsch el al (1990) Develop. 110, 1327-1340.
McCormack et al (1990) Proced. Nat. Acad. Sci. 87, 5227-5231.
Burch et al (1991) J. Clin. Invest. 88, 1190-1196.
Hampel and Tritz, Biochem. 28: 4929-4933 (1989), "RNA Catalytic Properties of the Minimum (-)sTRSV Sequence.".
Hampel et al., Nuc. Acids. Res. 18: 299-304 (1990), "`Hairpin` catalytic RNA model: evidence for helices and sequence requirements for substrate RNA.".
Fedor and Uhlenbeck, Proc. Natl. Acad. Sci. USA 87: 1668-1672 (1990), "Substrate Sequence Effects on hammerhead RNA catalytic efficiency.".
Koizumi et al., FEBS 239: 285-288 (1988), "Cleavage of specific sites of RNA by designed ribozymes.".
Lehman and Joyce, Nature 361: 182-185 (1993), "Evolution in vitro of an RNA enzyme with altered metal dependence.".
Goodchild et al., Arch. Biochem. and Biophys. 263: 401-409 (1988), "Inhibition of Rabbit .beta.-Globin Synthesis by Complementary Oligonucleotides: Identification of mRNA Sites Sensitive to Inhibition".
Uhlenbeck, Nature 328: 596-600 (1987), "A small catalytic oligoribonucleotide".
Kutyavin, et al., 1988, FEBS 238: 35-38, "N-2-Hydroxyethyl)phenazinium derivatives of oligonucleotides as effectors of the sequence-specific modification of nucleic acids with reactive oligonucleotide derivatives".
Maher and Dolnick, 1988, Nucleic Acids Res. 16: 3341-3358, "Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleoside methylphosphonates in a cell-free system".
Goodchild and Kohli, 1991, Archives of Biochem. & Biophysics 284: 386-391, "Ribozymes that cleave an RNA sequence from human immunodeficiency virus: the effect of flanking sequence on rate".
Sproat, et al., 1990, Nucleic Acids Res. 18:41-49, "New synthetic routes to protected purine 2'-O-methylriboside-3'-O-phosphoramidites using a novel alkylation procedure".
Goodchild, J., 1992, Nucleic Acids Res. 20: 4607-4612, "Enhancement of ribozyme catalytic activity by a contiguous oligodeoxynucleotide (facilitator) and by 2-'O-methylation".
LeGuyader John L.
Worcester Foundation for Experimental Biology
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