Method for the extraction of genomic DNA from fish blood or sper

Details Method for the extraction of genomic DNA from fish blood or sper Method for the extraction of genomic DNA from fish blood or sper

1995-09-27

2000-03-14

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2541, C07H 100, C07H 2104

Patent

active

060374640

ABSTRACT:
A simple and efficient process for the extraction of genomic DNA from a fish, requiring a minuscule amount of blood or sperm from the fish without sacrificing it, comprises: adding blood or semen taken from the fish into a first buffer for cell-lysis to create a first suspension; centrifuging the first suspension to collect a precipitated nuclear pellet; adding the precipitated pellet into a second buffer for nuclear-lysis to create a second suspension; and extracting DNA from the second suspension.

REFERENCES:
Brem et al., Aquaculture, 68: 209-219, 1988.
Dunham et al., Trans. Am. Fish Soc., 116: 87-91, 1987.
Ivics et al., Mol. Marine Biol. Biotechnol., 2: 162-173, 1993.
Khoo et al., Aquaculture, 107: 1-19, 1992.
Penman et al., Aquaculture, 85: 35-50, 1990.
Penman et al., Mol. Repr. Dev., 30: 201-206, 1991.
Zhang et al., Mol. Repr. Dev., 25: 3-13, 1990.
Taggart et al., J. Fish Biol. 40: 963-965, 1992.
Cummings et al., Biotechniques, 17(3): 426-430, 1994.

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