Thermostable DNA polymerase from Thermus thermophilus HB-8

Details Thermostable DNA polymerase from Thermus thermophilus HB-8 Thermostable DNA polymerase from Thermus thermophilus HB-8

1992-06-05

1995-05-09

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C12N 912

Patent

active

054139260

ABSTRACT:
A DNA polymerase from Thermus thermophilus which retains at least 60% of its activity when placed at pH 8.0 and 85.degree. C. for 2 hours is disclosed. The DNA polymerase has a pH optimum of about 8.0, a temperature optimum of about 75.degree. C., a mass of about 85 to 95 kDa and an optimal saline concentration of about 20-60 mM NaCl.

REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
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R. K. Saiki et al., "A Novel Method for the Detection of Polymorphic Restriction Sites by Cleavage of Oligonucleotide Probes: Application to Sickle-Cell Anemia," Bio/Technology, 3, 1008-1012 (1985).
A. Chien et al., "Deoxyribonucleic Acid Polymerase from the Extreme Thermophile Thermus acquaticus," Journal of Bacteriology, vol. 127, No. 3, pp. 1550-1557 (Sep. 1976).
Stoesz et al., "Refolding Transition of .alpha.-Chymotrypsin: pH and Salt Dependence," Biochemistry, 17(18), 3693-3699 (1978).
Hon-nami et al., "Denaturation of Thermophilic Ferricytochrome c-552 by Acid, Guanidine Hydrochloride, and Heat," Biochemistry, 18(25), 5693-5697 (1979).
Elie et al., "Thermostable DNA Polymerase from the archaebacterium Sufolobus acidocaldarius; Purification, characterization and immunological properties," Eur. J. Biochem., 178, 619-626 (1989).
Bechtereva et al., "DNA sequencing with thermostable Tet DNA polymerase from Thermus thermophilus," Nucleic Acids Res., 17(24), 10507 (1989).
Innis et al., "A Guide to Methods and Applications," PCR Protocol: A Guide To Methods And Appl., 129-141, Acad. Press, San Diego, Calif. (1990).

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