Method to predict tumor response to therapy

Details Method to predict tumor response to therapy Method to predict tumor response to therapy

1991-04-26

1993-12-14

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 2, 435 32, 4352402, 436501, C12Q 102, C12Q 118, A01N 102, C12N 500

Patent

active

052701727

ABSTRACT:
A method is disclosed for predicting response of a tumor patient to therapy, and selecting appropriate therapy for malignant neoplasms such as breast, ovarian and gastrointestinal cancer. A sample of tumor cells is cultured in the presence of 10 .mu.g/ml estradiol, and inhibition of cell growth in the culture paradoxically indicates an estrogen dependent tumor that will respond to antiestrogenic therapy. Another sample of tumor cells is cultured in the presence of a chemotherapeutic agent which predicts response of the tumor to in vivo administration of a cytotoxic agent. The chemotherapeutic agent is preferably a cytotoxic drug, or a drug having both cytotoxic and antiestrogenic mechanisms of action. A particularly suitable substance having both mechanisms of action is: ##STR1## wherein R.sup.1 H, OH, OOC(CH.sub.2).sub.2 CO.sub.2 H or CH.sub.3 COO; R.sup.2 is C.sub.6 H.sub.4 OH, C.sub.6 H.sub.4 OCOCH.sub.3, C.sub.6 H.sub.4 OOC(CH.sub.2)CO.sub.2 H, or C.sub.6 H.sub.5 ; and X is C.sub.6 H.sub.3 -2,4(NO.sub.2).sub.2, C.sub.6 H.sub.5, C.sub.6 H.sub.4 -4(NO.sub.2), C.sub.6 H.sub.4 -3(NO.sub.2), or C.sub.6 H.sub.3 -2,4(NO.sub.2).sub.2 ;
Predictive power of the assay is increased by combining information from the estradiol and chemotherapeutic assays. These assays are preferably performed in culture flasks having removable bases that function as conventional microscope slides.

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