Method of maintaining a desired recombinant gene in a genetic po

Details Method of maintaining a desired recombinant gene in a genetic po Method of maintaining a desired recombinant gene in a genetic po

1995-06-07

1998-11-24

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352523, 43525233, 4353201, C12Q 168

Patent

active

058404830

ABSTRACT:
The invention encompasses methods of maintaining desired recombinant genes in a genetic population of cells expressing the recombinant gene. The methods utilize mutant cells which are characterized by a lack of a functioning native gene encoding an enzyme which is essential for cell survival, wherein this enzyme catalyzes a step in the biosynthesis of an essential cell wall structural component and the presence of a first recombinant gene encoding an enzyme which is a functional replacement for the native enzyme, wherein the first recombinant gene cannot replace the defective chromosomal gene. The first recombinant gene is structurally linked to a second recombinant gene encoding a desired product. Loss of the first recombinant gene causes the cells to lyse when the cells are in an environment where a product due to the expression of the first recombinant gene is absent. The invention also encompasses methods of creating and isolating mutant cells with the above characteristics. The cells of the invention are useful for commercial production of desired products, for components of vaccines for immunizing individuals, and for release into the environment.

REFERENCES:
patent: 5387744 (1995-02-01), Curtiss, III et al.

Affiliated with

Also associated with

Rating

Method of maintaining a desired recombinant gene in a genetic po does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of maintaining a desired recombinant gene in a genetic po, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of maintaining a desired recombinant gene in a genetic po will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1700548