Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1989-10-30
1991-12-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 435 91, 4351723, 436501, 536 27, 935 6, 935 10, 935 29, 935 36, 935 77, 935 78, 935 88, C12Q 168
Patent
active
050700103
ABSTRACT:
The anti-viral transactivating activity of a test substance is determined by (a) putting both the expression of the Secreted Alkaline Phosphatase gene and the viral transactivator gene under the control of a viral promoter responsive to the action of the transactivator, (b) transfecting cultured mammalian cells with plasmids which contain the gene constructs of (a) and cause cellular production of the transactivating factor and Secreted Alkaline Phosphatase (c) adding the substance to be tested, and (d) determining the amount of Secreted Alkaline Phosphatase produced, whereby inhibition of Secreted Alkaline Phosphatase production correlates with anti-viral transactivating activity.
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Gillespie, Molecular and Cellular Probes 3:73-86 (1989).
Epstein William H.
Gould George M.
Hoffman-La Roche Inc.
Marschel Ardin H.
Rocha Patricia S.
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