DNA fragment and expression plasmid containing the DNA fragment

Details DNA fragment and expression plasmid containing the DNA fragment DNA fragment and expression plasmid containing the DNA fragment

1989-12-08

1993-07-06

Lacey, David L.

Chemistry: molecular biology and microbiology

Vector, per se

536 241, 435 691, 4351723, C12N 1563, C12N 1512, C07H 2104

Patent

active

052253481

ABSTRACT:
A DNA fragment containing a promoter region for human polypeptide chain elongation factor-1.alpha., its base sequence and expression plasmids containing the DNA fragment having high applicability to a wide range of host cells with high expression capacity. These expression plasmids can be maintained stably for at least one month in mammalian host cells. Such features of the expression plasmids may render possible the efficient production of various kinds of useful physiologically active substances for a long period using wide range of mammalian cells as the host.

REFERENCES:
Mizushima and Nagata, Nucleic Acids Research 18: 5322 (1990).
Uetsuki, et al. Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1.alpha.*; J. Biol. Chemistry vol. 264, No. 10, pp. 5791-5798 Apr. 5, 1989.

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