Situ hybridization slide processes

Details Situ hybridization slide processes Situ hybridization slide processes

1996-10-09

2000-02-08

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

060226897

ABSTRACT:
A method of preparing flourescent in situ hybridization slides, comprising: providing a slide with a raised ring thereon; providing to the slide a DNA probe; providing to the slide cells and target DNA; providing to the slide a formamide-free mixture of 10%.+-.2% by weight dextran sulfate and 15%-25% glycerol and 0.9% by weight salt or a solution of 10%.+-.2% by weight dextran sulfate, 10-30% by volume formamide and 0.9% by weight salt; placing a coverglass on the ring; denaturing and hybridizing the slide; and performing a post-hybridization wash of the slide.

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