Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1987-12-21
1990-08-21
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435251, 435258, 435947, 436 30, C12Q 118, C12Q 102, C12R 190
Patent
active
049505947
ABSTRACT:
A microbiological assay based on bioluminesce employing the bioluminescent dinoflagellate Pyrocystis lunula. An oil well drilling fluid sample is prepared according to E. P. A. procedures to obtain a suspended particulate phase sample. An aliquot of the sample is added to a growth medium containing Pyrocystis lunula in suspension. The mixture is agitated to subject the Pyrocystis lunula to a shear stress. Light emitted as a result of the shear stress on the Pyrocystis lunula is measure and compared with a control to determine if there is diminution of light produced by the Pyrocystis lunula in the mixture. Diminution of light production is an indication of the presence of a toxic substance in the sample.
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Bulich, "Bioluminescence Assays", Chapter 4, 57-74, in Bitton and Dutka (eds.), Toxicity Testing Using Micro Organisms, vol. 1, CRC Press (1986).
Kepplinger Esther L.
McDonald Thomas E.
Phillips Thomas M.
Scheiner Toni R.
The United States of America as represented by the Secretary of
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