Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds

Details Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds

1996-03-07

1999-11-30

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 536 266, C12Q 168

Patent

active

059940638

ABSTRACT:
The use of BODIPY.RTM. fluorophores for detecting a target nucleic acid is described. The parent heterocyclic molecule of the BODIPY.RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present invention provides oligonucleotides labelled with substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene (BODIPY.RTM. fluorophore) compounds for performing the Taqman assay.

REFERENCES:
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5274113 (1993-12-01), Kang et al.
patent: 5326692 (1994-07-01), Brinkley et al.
patent: 5491063 (1996-02-01), Fisher et al.
patent: 5538848 (1996-07-01), Livak et al.
Holland et al., Detection of Polymerase Chain Reaction . . . PNAS, 88:7276-80 (1991).
Lee et al., Allelic discrimination by nick-translation PCR with fluorogenic probes, Nucleic Acid Res. 21:3761-66 (1993).

Affiliated with

Also associated with

Rating

Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1669955