Nucleoside triphosphate-dependent 1-methylhydantoinase, a proces

Details Nucleoside triphosphate-dependent 1-methylhydantoinase, a proces Nucleoside triphosphate-dependent 1-methylhydantoinase, a proces

1985-02-25

1989-03-28

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 25, 435 26, 435195, 435231, 435810, C12Q 134, C12Q 126, C12Q 132, C12N 914, C12N 986

Patent

active

048163937

ABSTRACT:
The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.
The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.
Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination

REFERENCES:
patent: 3907644 (1975-09-01), Mollering et al.
patent: 4087329 (1978-05-01), Terada et al.
patent: 4134793 (1979-01-01), Terada et al.
patent: 4215197 (1980-07-01), Tarbutton
patent: 4645739 (1987-02-01), Deeg et al.
Yamada et al, Chemical Abstracts, 104:105830q (1986), FEMS Microbiol. Lett., 30(3):337-340 (1985).

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