Immunochemical assay of carcinoembryonic antigen and reagent the

Details Immunochemical assay of carcinoembryonic antigen and reagent the Immunochemical assay of carcinoembryonic antigen and reagent the

1985-04-10

1989-03-28

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 28, 435810, 436518, 436813, G01N 3353

Patent

active

048163902

ABSTRACT:
An immunochemical assay of human carcinoembryonic antigen (CEA), comprising the use of an antibody supported on a carrier and an antibody labelled with peroxidase by coupling by means of a compound of the formula: ##STR1## wherein n is an integer of 0 to 5 and R is a chemical bond or a divalent 6-membered cyclic hydrocarbon residue, in which at least one of said antibodies is a monoclonal antibody. The assay can be conducted with high sensitivity by simple and easy operation in clinical laboratories.

REFERENCES:
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Ishikawa et al., J. Immunoassay, vol. 4, No. 3, (1983), pp. 209-265.
Krupey et al., Immunochemistry, vol. 9, (1972), pp. 617-622.
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Carrico et al., Cancer Res., vol. 35, (1975), pp. 2928-2934.
Yoshitake et al., Analytical Letters, vol. 15, (1982), pp. 147-160.
Ishikawa et al.; Journal of Immunoassay 4, pp. 250-264 (1983).
Kitagawa et al., J. Biochem., 79, 233-236 (1976).
Yoshitake et al., Eur. J. Biochem., 101, 395-399 (1979).

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