Enzyme amplification compounds for assays for androgens

Details Enzyme amplification compounds for assays for androgens Enzyme amplification compounds for assays for androgens

1980-12-30

1983-03-15

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

G01N 3114, L07G 702

Patent

active

043768253

ABSTRACT:
Novel biological assay method for determining the presence of a specific organic material by employing a modified enzyme for amplification. By employing receptors specific for one or a group of materials (hereinafter referred to as "ligands") and binding an enzyme to the ligand or ligand counterfeit to provide an "enzyme-bound-ligand", an extremely sensitive method is provided for assaying for ligands. The receptor when bound to the enzyme-bound-ligand substantially inhibits enzymatic activity, providing for different catalytic efficiencies of enzyme-bound-ligand and enzyme-bound-ligand combined with receptor.
The receptor, ligand and enzyme-bound-ligand are combined in an arbitrary order and the effect of the presence of ligand on enzymatic activity determined. Various protocols may be used for assaying for enzymatic activity and relating the result to the amount of ligand present.

REFERENCES:
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 4191613 (1980-03-01), Ullman et al.

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