Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-01-27
1998-07-21
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435190, 435225, 4352543, 4353201, 536 232, 536 241, C12P 2102
Patent
active
057834148
ABSTRACT:
The invention relates to an expression system which is usable for the extracellular production of glucose oxidase, which comprises the sequence of a promoter, the glucose oxidase secretion signal sequence, the mature glucose oxidase sequence and the sequence of a terminator. The invention also relates to an integration vector containing the expression system, and to a cell transformed by this vector.
REFERENCES:
patent: 5094951 (1992-03-01), Rosenberg
patent: 5266688 (1993-11-01), Rosenberg
patent: 5360732 (1994-11-01), Berka et al.
patent: 5360901 (1994-11-01), Berka et al.
Verdoes et al. "The Effect of Multiple Copies of the Upstream Region on Expression of The Aspergillus niger Glucoamylase-Encoding Gene" Gene 145 179-187 1994.
Hodgkius et al. "Expression of The Glucose Oxylase Gene from Aspergillus niger. . . " Yeast 9 625-635 1993.
De Baetseller et al. Fermentation of a Yeast Producing A. Niger Gluclose Oxidase Bio/Technol. 9 559-561 1991.
Applied and Enviromental Microbiology, Apr. 1992, pp. 1190-1194, vol. 58, No. 4, Cor F.B. Witteveen, et al., "Localization of Glucose Oxidase and Catlase Activities in Aspergillus Niger".
Curr. Genet., 1990, vol. 18, No. 531, pp. 531-536, H. Whittington, et al., "Expression of the Aspergillus Niger Glucose Oxidase Gene in A. Niger, A. Nidulans and Saccharomyces Cerevisiae".
Analytical Biochemistry, vol. 135, pp. 416-422, 1983, Robert C. Garber, et al., "Isolation of DNA From Filamentous Fungi and Separation into Nuclear, Mitochondrial, Ribosomal, and Plasmid Components".
Notice/kit: "Geneamp DNA Amplification Reagent Kit With Amplitaq Recombinant Taq DNA Polymerase", pp. 1-5.
Science, vol. 239, Jan. 29, 1988, Randall K. Saiki, et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase", pp. 487-491.
Tetrahedron Letters, vol. 22, No. 20, pp. 1859-1862, 1981, S.L. Beaucage, et al., "Deoxynucleoside Phosphoramidites--A New Class of Key Intermediates for Deoxypolynucleotide Synthesis".
Molecular Cloning--a laboratory manual, T. Maniatis, et al., 1982, pp. 374-379, "Constructing Maps of Sites Cleaved By Restriction Endonucleases".
The Journal of Biological Chemistry, vol. 265, No. 7, Mar. 1990, pp. 3793-3802, Katherine R. Frederick, et al., "Glucose Oxidase From Aspergillus Niger".
Molecular Cloning--a laboratory manual--second edition, 1989, J. Sambrook, et al., pp. 1.68-1.69; pp. 5.28-5.32; and pp. 9.52-9.55.
Current Protocols in Molecular Bioogy, F.M. Ausubel, 1989, "Size Fractionation Using Sucrose Gradients", pp. 5.3.2-5.3.8.
J. Mol. Biol., 1983, vol. 166, pp. 557-580, Douglas Hanahan, "Studies on Transformation of Escherichia Coli with Plasmids".
Bethesda Research Laboratories, (BRL), 1986, Focus vol. 8, No. 2, p. 9, "Tools for the Molecular Biologist".
The EMBO Journal, vol. 3, No. 5, pp. 1097-1102, 1984, E. Boel, et al., "Glucoamylases G1 And G2 From Aspergillus Niger are Synthesized From Two Different But Closely Related mRN As".
Proc. Natl. Acad. Sci. USA, vol. 74, No. 12, pp. 5463-5467, Dec. 1977, F. Sanger, et al., "DNA Sequencing With Chain-terminating Inhibitors".
Notice/kit (BIORAD), "Muta-gene Phagemid In Vitro Mutagenesis Kit", Instruction Manual, pp. 1-26.
Bio/Technology, vol. 3, Nov. 1985, Jingdong Zhu, et al., "A Method for Fast and Pure DNA Elution from Agarose Gels by Centrifungal Filtration", pp. 1012, 1015-1016.
Advances in Genetics, vol. 5, 1953, pp. 141-238, G. Pontecorvo, et al.,"The Genetics of Aspergillus Nidulans".
Gene, vol. 94, 1990, pp. 147-154, Dirk Carrez, et al., "Heterologous Gene Expression by Filamentous Fungi: Secretion of Human Interleukin-6 by Aspergillus Nidulans".
Molecular and Cellular Biology, Aug. 1983, vol. 3, No. 8, pp. 1430-1439, Michael J. Hynes, et al., "Isolation of Genomic Clones Containing the amdS Gene of Aspergillus Nidulans and their use in the Analysis of Structural and Regulatory Mutations".
The EMBO Journal, vol. 4, No. 2, pp. 475-479, 1985, Joan M. Kelly, et al., "Transformation of Aspergillus Niger by the amdS Gene of Aspergillus Nidulans".
Enzyme Microb. Technol., Dec. 1986, vol. 8, Jan Fiedurek, et al., "Screeing and Mutagenesis of Moulds for the Improvement of Glucose Oxidase Production", pp. 734-736.
Molecular Cloning--a laboratory manual--second edition, J. Sambrook, et al., 1989, pp. 1.25-1.28; 1.63-1.73; 1.75; 6.22-6.35; 9.24.
Molecular Cloning--a laboratory manual--second edition, J. Sambrook, et al., 1989, pp. 13.45-13.58.
Molecular Cloning--a laboratory manual--second edition, 1989, J. Sambrook, et al., pp. 13.14-13.17.
J. Cell. Biol., No. 10, p. 274, Feb. 1986, K. Hayenga, et al., "Expression and Secretion of Bovine Calf Chymosin by Aspergillus Nidulans".
Appl. Biochem. Microbiol., vol. 14, No. 3, p. 369, 1978, S.A.Z. Mahmoud, et al., "Isolation and Purification of a Glucoamylase Preparation from a Submerged Culture of Aspergillus Foetidus ATCC 14916".
Applied Microbiology, vol. 25, No. 6, pp. 890-895, Jun. 1973, L. T. Chang, et al., "Intergenic Complementation of Glycoamylase and Citric Acid Production in Two Species of Aspergillus".
Carrez Dirk
Roos Joel
Degen Nancy
Genencor International Inc.
LandOfFree
Expression system, integration vector and cell transformed by th does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression system, integration vector and cell transformed by th, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression system, integration vector and cell transformed by th will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1646045