Method for the enhancement of cell images

Image analysis – Histogram processing – For setting a threshold

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382 54, 359370, G06K 954

Patent

active

053176448

ABSTRACT:
The present invention relates to an image processing method for enhancing the digitised optical image section of a (e.g. substantially transparent biological) specimen and in a particular aspect the boundary outline of a specimen in such an image section thereof. In accordance with the invention a Nomarski image section is smoothed and a gradient of image pixel intensity determined for each pixel thereof. Thereafter each pixel of the smoothed Nomarski image which has an intensity gradient within a reference range is set to zero intensity so as to provide a processed image section.

REFERENCES:
patent: 4873653 (1989-10-01), Grosskopf
patent: 4884225 (1989-11-01), Fogarty
patent: 4932044 (1990-06-01), Williams et al.
patent: 4991224 (1991-02-01), Takahashi et al.
patent: 5020113 (1991-05-01), Lo et al.
patent: 5119444 (1992-06-01), Nishihara
Improvement in the resolution of three-dimensional data sets collected using optical serial sectioning, F. Macias Garza, et al, 1989 Journal of Microscopy, vol. 153 Pt 2 Feb. 1989 pp. 205-221.
Confocal differential interference contrast (DIC) microscopy; including a theoretical analysis of conventional and confocal DIC imaging, C. J. Cogswell et al, Journal of Microscopy, vol. 165 Pt 1, Jan. 1992, pp. 81-101.
Extracting Geometric Models Through Constraint Minimization, (Miller J. A., Breen D. E., Wozny M. J., IEEE, 1990, CH2913-2.
The Nomarski interference-contrast microscope. An experimental basis for image interpretation. J. Padawer. Journal of The Royal Microscopic Society, 1968, vol. 88, part 3, pp. 305 to 349.
Gonzalez & Wintz, Digital Image Processing, Wiley, 1976, pp. 160-175.
Handbook of Biological Confocal Microscopy, James B. Pawley, Revised Edition, Plenum Press N.Y. and London, pp. 41-51.
New Edge detection methods on exponential filter, Castan, S. Zhao, J. Shen J. Proceedings 10th International Conference on Pattern Recognition (Cat. No. 90CH2898-5) pp. 709 to 711 vol. 1.
Wither Video Microscopy. Towards 4-D imaging at the Highest Resolution of the Light Microscope, Shinya Inoue, Optical Microscopy for Biology, pp. 497-511, 1990 Wiley-Liss Inc.

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