Process for purifying elastase

Details Process for purifying elastase Process for purifying elastase

1982-12-01

1984-07-03

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435814, C12N 966

Patent

active

044580185

ABSTRACT:
A process is disclosed for purifying elastase by precipitating elastase from an aqueous, elastase-containing solution, in which the electroconductivity of said aqueous solution is adjusted to 3 millimhos per centimeter or less and the pH of the solution is adjusted to a value of from 2 to 5. The electroconductivity of said aqueous solution is preferably adjusted to 3 millimhos per centimeter or less by means of ultrafiltration.

REFERENCES:
patent: 4071410 (1978-01-01), Yoshizawa
Lewis et al., Journal of Biological Chemistry, vol. 256, pp. 705-720, (1956).
Methods in Enzymology, vol. 19, pp. 113-140, (1970).

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