Pullulanase, methods of producing pullulanase and methods of sac

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

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435 98, 435210, C12P 2100, C12P 1916, C12N 944

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active

053169247

ABSTRACT:
The present invention provides a novel pullulanase, methods of producing and isolating the pullulanase and methods of saccharifying starch using the pullulanase. The pullulanase of the invention works in a wide pH range, as large as pH 4-9. In addition, the pullulanase is heat stable and its optimum temperature is about 60.degree. C. Therefore, the pullulanase of the invention can be used in the saccharification of starch along with amylase, which is enzymatically active at pH 4-9, to increase the yield of saccharides.

REFERENCES:
patent: 3560345 (1971-02-01), Yokobayashi et al.
patent: 3565765 (1971-02-01), Heady et al.
patent: 3654082 (1972-04-01), Abdullah
patent: 3716455 (1973-02-01), Ueda et al.
patent: 3741873 (1973-06-01), Ueda et al.
patent: 3790446 (1974-02-01), Gunja-Smith
patent: 3827940 (1974-08-01), Sugimoto et al.
patent: 3963575 (1976-06-01), Bulich
M. Abdullah and D. French, Arch. Biochem. Biophys., 137: 483-493 (1970), "Substrate Specificity of Pullulanase".
K. Kainuma et al., Biochem. Biophys. Acta, 410: 333-346 (1975), "Purification and Some Properties of a Novel Maltohexose-Producing Exo-Amylase from Aerobacter Aerogenes".
B. E. Norman, Starch/Starke 34: 340-346 (1982), "A Novel Debranching Enzyme for Application in the Glucose Syrup Industry".
J. F. Robyt and R. J. Ackerman, Arch. of Biochem and Biophys., 145: 105-114 (1971); "Isolation, Purification and Characterization of a Maltotetraose-Producing Amylase from Pseudomonas stutzeri".
N. Saito, Arch. of Biochem. and Biophys., 155: 290-298 (1973), "A Thermophilic Extracellular .alpha.-Amylase from Bacillus lichenformis ".
Y. Takasaki, Agr. Biol. Chem., 40 (8): 1523-1530 (1976), "Purifications and Enzymatic Properties of .beta.-Amylase and Pullullanase from Bacillus cerus var. mycoides".
Y. Takasaki, Agric. Biol. Chem., 46 (6): 1539-1547 (1982) "Production of Maltohexaose by .alpha.-Amylase from Bacillus Circulans G-6".
Y. Takasaki, Agric. Biol. Chem., 49(4): 1091-1097 (1985), "An Amylase Producing Maltotriose from Bacillus subtilis".
Y. Takasaki, Agric. Biol. Chem., 51 (1): 9-16 (1987)"Pullulanase-Amylase Complex Enzyme from Bacillus subtilis".
Y. Takasaki et al., Agric. Biol. Chem., 55 (7): 1715-1720 (1991), "Maltotetraose-producing Amylase from Bacillus sp. MG-4".
Y. Takasaki et al., Agric. Biol. Chem., 55 (3): 687-692 (1991), "Maltotriose-producing Amylase from Microbacterium imperiale".
S. Ueda and N. Nanri, Applied Microbiol., 15 (3): 492-499 (1967), "Production of Isoamylase by Escherichia intermedia".
S. Ueda et al., J. Ferment Technol., 49 (6): 552-558 (1971), "Production of Isoamylase by Streptomyces sp. No. 28".
G. J. Walker, Biochem. J., 108: 33-40 (1968), "Metabolism of the Reserve Polysaccharide of Streptococcus mitis".

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