Production and use of monoclonal antibodies to phosphotyrosine-c

Details Production and use of monoclonal antibodies to phosphotyrosine-c Production and use of monoclonal antibodies to phosphotyrosine-c

1982-12-13

1985-09-24

Padgett, Ben R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435240, 435948, 435 4, 435 7, 435 15, 435 68, 935104, 935108, 935110, 436514, 436518, 436536, 436537, 436539, 436540, 436541, 436542, 436548, 436804, 436813, 436815, 436821, 436823, 260112R, C12N 1500, G01N 3354, G01N 3356, G01N 3360

Patent

active

045434395

ABSTRACT:
A hybridoma cell line is disclosed that secretes monoclonal antibodies which serve as a high titer, reproducible, biological reagent useful in biological/medical research for isolating and identifying phosphotyrosine-containing proteins. In addition, the antibodies have potential uses in diagnosis of a variety of diseases, including certain cancers. The antibodies, which have demonstrated affinity for a variety of molecules containing o-phosphotyrosine residues, were prepared using a synthetic analog, p-azobenzyl phosphonate (ABP) covalently linked to a carrier protein, as the antigen.

REFERENCES:
Ross, A. H. et al., Nature, vol. 294, pp. 654-656 (1981).
Veronese, F. et al., J. Virology, vol. 4313, pp. 896-904 (9-1982).
Hunter, Tony & Sefton, B. M. "Transforming Gene Product of Rous Sarcoma Virus Phosphorylates Tyrosine", Proc. Natl. Acad. Sci., U.S.A., vol. 77, No. 3, pp. 1311-1315, Mar. 1980.
Oi, Vernon T. & Herzenberg, L. A., "Immunoglobulin-Producing Hybrid Cell Lines" Selected Methods in Cellular Immunology, Ed. Mishell & Shiigi, pp. 351-371, Pub. W. H. Freeman & Co., San Francisco, CA.
Ushiro, Hiroshi & Cohen, S. "Identification of Phosphotyrosine as a Product of Epidermal Growth Factor-Activated Protein Kinase in A-431 Cell Membranes: The Journal of Biological Chemistry, vol. 255, No. 18, Sep. 25, 1980, pp. 8363-8365.
Witte, Owen N., Dasqupta, A. & Baltimore, D., Nature, vol. 283, Feb. 28, 1980, pp. 826-831.

Affiliated with

Also associated with

Rating

Production and use of monoclonal antibodies to phosphotyrosine-c does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production and use of monoclonal antibodies to phosphotyrosine-c, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production and use of monoclonal antibodies to phosphotyrosine-c will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1615184