Differential hybridization for relative quantification of varian

Details Differential hybridization for relative quantification of varian Differential hybridization for relative quantification of varian

1996-07-29

1998-10-27

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 912, C12Q 170, C12Q 168, C12P 1934

Patent

active

058276483

ABSTRACT:
This invention relates to a method of measuring the relative populations of first and second variants of a target nucleotide sequence of a target genome in a sample utilizing an amplification step, followed by probing with first and second variant probes and a control probe. Specific embodiments include an assay to monitor the conversion of codon 215 for HIV-1 reverse transcriptase from wild type to mutant form.

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patent: 5631128 (1997-05-01), Kozel et al.
Gingeras et al. Jour. Infect. Dis. 164: 1066-1074, 1991.
Rudert et al. Nuc. Acids Reg. 20(5): 1106, 1992.

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