Regeneration of cotton plant in suspension culture

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se

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4354301, 435431, A01H 400, C12N 504

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056959990

ABSTRACT:
A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.

REFERENCES:
Finer et al. 1984, Plant Cell Reports 3:41-43.
Shoemaker et al. 1986, Plant Cell Reports 5(3):178-181.
Smith et al., 1977, In Vitro 13(5):329-334.

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