Process for production of D-.alpha.-amino acids

Details Process for production of D-.alpha.-amino acids Process for production of D-.alpha.-amino acids

1995-06-07

1997-12-09

Jagannathan, Vasu S.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435 43, 435 691, 435 712, 435176, 435195, 435227, 435228, 4352523, 4353201, 536 221, 536 231, 536 232, 536 237, C12P 1304, C12P 2106, C12N 120, C07H 1900

Patent

active

056959680

ABSTRACT:
The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme.
The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.

REFERENCES:
Olivieri et al., "Microbial Transformation of Racemic Hydantoins to D-Amino Acids", Biotechnology and Bioengineering 23:2173-2183 Oct. 1981.

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