Eukaryotic expression vector system

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 435 9121, 435 914, 435 9141, 435 915, 4353201, 4351723, 435975, 536 231, 536 235, 536 241, 536 253, 935 2, 935 9, 935 22, 935 32, 935 80, C12P 1934

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056041187

ABSTRACT:
The present invention relates to two plasmid vectors and eucaryotic expression vector created therefrom. The present invention further relates to a method of cloning a gene in a eucaryotic cell the expression of which is affected by drug treatment, a method of constructing a subtracted cDNA library and a method of identifying a eucaryotic gene the product of which inhibits cell growth.

REFERENCES:
patent: 5238834 (1993-08-01), Pruitt
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Maniatis et al. 1989 in Molecular Cloning. A Laboratory Manual. Second Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. pp. 10.3 and 10.6.
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Forster et al. 1985. Nuc. Acids Res. 13, 745-761.
Sambrook et al. (eds.) 1989 in: Molecular Cloning. A Laboratory Manual, Second edition. Cold Spring Harbor, New York, pp. 8.48-8.49, 10.40-10.43.
Asubel et al 1988. Current Protocols in Molecular Biology. vol. 2, John-Wiley & Sons., N.Y. pp. 12.6.1-12.6.9.
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Pharmacia LKB Molecular Biology Products 1988 Catalog.
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Margolskee et al. 1988 Molec. Cell. Biol. 8, 2837-2847.
Duguid et al. 1988 Proc. Natl. Acad Sci. USA. 85, 5738-5742.

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