Method of identifying compounds affecting hedgehog cholesterol t

Details Method of identifying compounds affecting hedgehog cholesterol t Method of identifying compounds affecting hedgehog cholesterol t

1997-10-07

2000-05-02

Spector, Lorraine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

530350, 530300, 436 71, C07K 1447, C07K 14475, G01N 3392

Patent

active

060570911

ABSTRACT:
The present invention provides two novel polypeptides, referred to as the "N" and "C" fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G.sup..dwnarw. CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog protein are described.

REFERENCES:
patent: 5143830 (1992-09-01), Holland et al.
patent: 5789543 (1998-08-01), Ingham et al.
patent: 5844079 (1998-12-01), Ingham et al.
Clarke et al., "Cellular Lipid binding proteins: expression, function and nutritional regulation," FESEB J., vol. 3, pp. 2480-2487, Nov. 1989.
Mohler et al., "Molecular organization and embryonic expression of the hedgehog gene involved in cell-cell communication in segmental patterning of Drosophila," Development, vol. 115, pp. 957-971, 1992.
Lee et al., "Secretion and Localized transcription suggest a role in positional signaling for products of the segmentation gene hedgehog", Cell, vol. 71, pp. 33-50, Oct. 2, 1992.
Placzek, "Induction of floor plate differentiation by contact-dependent, homogenetic signals," Development, vol. 117, pp. 205-218, 1993.
Krauss et al., "A Functionally Conserved Homolog of the Drosophila Segment Polarity Gene hh Is Expressed in Tissues with Polarizing Activity in Zebrafish Embryos", Cell, vol. 75, pp. 1431-1444, Dec. 31, 1993.
Echelard, et al., "Sonic Hedgehog, a Member of a Family of Putative Signaling Molecules, Is Implicated in the Regulation of CNS Polarity," Cell, vol. 75, pp. 1417-1430, Dec. 31, 1993.
Ngo, et al., "Computational Complexity, Protein Structure Prediction and Levinthal Paradox", The Protein Folding Problem and Tertiary Structure Prediction, Merz, et al., eds., Birkhauser, Boston, pp. 433, 492-495, 1994.
Lee et al., "Autoproteolysis in hedgehog Protein Biogenesis", Science, vol. 266, pp. 1528-1537, Dec. 2, 1994.
Tabata and Kornberg, "Hedgehog is a Signaling Protein with a Key Role in Patterning Drosophila Imaginal Discs", Cell, vol. 76, pp. 89-102, Jan. 14, 1994.
Ingham, P.W., "Signaling by hedgehog family proteins in Drosophila and vertebrate development", Cur. Opin. Gen. Dev., vol. 5, pp. 492-498, 1995.
Hynes, et al., "Induction of Midbrain dopamenergic Neurons by Sonic Hedgehog," Neuron, vol. 15, pp. 35-44, Jul., 1995.
Farase et al., Cholesterol metabolism and embryogenesis, Trends in Genetics, 14(3):115-120, Mar. 1998.
Kolf-Clauw et al., Abnormal cholesterol biosynthesis as a Smith-Lemli-Opitz syndrome disrupts normal skeletal development in the rat, J. Lab. Clin. Med., 131:222-227, Mar. 1998.

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