Hydrolyzable compounds which release electron transfer agents an

Details Hydrolyzable compounds which release electron transfer agents an Hydrolyzable compounds which release electron transfer agents an

1987-06-08

1990-08-28

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 7, 435 14, 435 19, 435 21, 435 22, 435 23, 435 24, 558 37, 558194, 530408, 530409, 530410, 536 41, 536 174, 544 31, 544337, 544347, 552296, 552298, 548368, C12Q 134, C07D24146

Patent

active

049524950

ABSTRACT:
Hydrolyzable substrates comprise blocked moieties which, when cleaved from the substrate during hydrolysis, provide electron transfer agents. The released electron transfer agents can be recycled between a reductant and a reducible compound that upon reduction provides a detectable species. Alternatively, they can be recycled between an oxidant and an oxidizable compound that upon oxidation provides a detectable species. These substrates are useful in analytical compositions, elements and methods for the determination of hydrolytic analytes, such as hydrolytic enzymes or biological cells containing such enzymes.

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Shiseido-II, Abstract of German Patent 3510482, 8/28/86.
Duerckheimer et al., Index Chemicus, 17, #50297, 1965.

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