Steroid-delta-isomerase

Details Steroid-delta-isomerase Steroid-delta-isomerase

1975-04-09

1976-04-06

Shapiro, Lionel M.

Textiles: fiber preparation

195 66R, 1951035R, 195 51R, C07G 7028, C12D 1310, C12K 100

Patent

active

039487288

ABSTRACT:
The new enzyme steroid-.DELTA.-isomerase is provided, as well as a process for producing same, comprising digesting a micro-organism containing said isomerase, extracting same by destruction of the cell wall with a buffer solution containing a non-ionic surfactant, centrifuging the resulting mass and discarding the precipitate, applying the extract to an anion exchanger, eluting the enzyme with a buffer solution containing a non-ionic surfactant, heating the eluate, separating the precipitate and isolating the enzyme therefrom from the supernatant.

REFERENCES:
alfsen et al., Biochem. and Biophys. Res. Comm., Vol. 20, pp. 251-255, (1965).

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