Methods and materials for obtaining microbial expression of poly

Details Methods and materials for obtaining microbial expression of poly Methods and materials for obtaining microbial expression of poly

1982-12-01

1987-05-19

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351723, 435 68, 435 70, 435317, 536 27, 935 8, 935 10, 935 16, 935 17, 935 18, C12P 1934, C12P 2100, C12P 2102, C12N 1500, C12N 100, C07H 1700

Patent

active

046668390

ABSTRACT:
Disclosed are methods for selective modification of double-stranded DNA sequences facilitating their storage and incorporation into expression vectors. Manufactured partially double-stranded DNA primers are hybridized to complementary regions of single-stranded forms of DNA sequences to be altered. Desired, selectively modified double-stranded DNA sequences are then formed by DNA polymerization and specific nuclease digestion of undesired double- and single-stranded regions. Illustratively provided are DNA sequences useful in the microbial expression of bovine prolactin and other valuable polypeptides such as avian growth hormone.

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