Probes labelled with energy transfer coupled dyes

Details Probes labelled with energy transfer coupled dyes Probes labelled with energy transfer coupled dyes

1995-12-19

1997-11-18

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, C12Q 168

Patent

active

056886489

ABSTRACT:
Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

REFERENCES:
patent: 4996143 (1991-02-01), Heller et al.
patent: 5188934 (1993-02-01), Menchen et al.
patent: 5326692 (1994-07-01), Brinkley et al.
patent: 5342789 (1994-08-01), Chick et al.
Parkurst and Parkhurst, Biochemistry 34:293-300, Jan. 1995.
Parkhurst and Parkhurst, Biochemistry 34:285-292, Jan. 1995.
McKeown et al. (1994) BioTechniques 17:901-7, Jan. 1995.
Benson et al. (1993) Nucleic Acids Research 21:5720-26.
Fregeau and Fourney (1993) BioTechniques 15:100-119.
Hiyoshi and Hosoi (Sep. 1994) Anal. Biochem. 221:306-11.
Frey (1994) Cytometry 17:310-18.
Sixou et al. (Feb. 25, 1994) Nucleic Acids Research 22:662-8.
Wei et al. (1994) Anal. Biochem. 66:1500-6.
Ansorge et al., "Automated DNA Sequencing: Ultrasensitive Detection of Fluorescent Bands During Electrophoresis," Nucleic Acids Research (1987), 15:4593-4602.
Cardullo et al., "Detection Nucleic Acid Hybridization by Nonradiative Fluorescence Resonance Energy Transfer," PNAS USA (1988), 85:8790-8794.
Clegg et al., "Fluorescence Resonance Energy Transfer Analysis of the Structure of the Four-Way DNA Junction," Biochemistry (1992), 31:4846-4856.
Huang et al., "DNA Sequencing Using Capillary Array Electrophoresis," Anal. Chem. (1992), 64:2149-2154.
Maliwal et al., "End-to-End Diffusion Coefficients and Distance Distributions from Fluorescence Energy Transfer Measurement: Enhanced Resolution by Using Multiple Acceptors with Different Forster Distances," Biophysical Chemistry (1993), 46:273-281.
Mathies and Huang, "Capillary Array Electrophoresis: An Approach to High-speed, High-thoughput DNA Sequencing," Nature (1992), 359:167-169.
Middenford et al., "Continuous, On-Line DNA Sequencing Using a Versatile Infrared Laser Scanner/Electrophoresis Apparatus," Electrophoresis (1992), 13:487-494.
Montenay-Garestier et al., "Design of Bifunctional Nucleic Acid Ligands," Molecular Basis of Specificity in Nucleic Acid-Drug Interactions, B. Pullman and J. Jortner (eds) (1990), 275-290.
Prober et al., "A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides," Science (1987), 238:336-341.
Ruth et al., "Linker Arm Nucleotide Analogs Useful in Oligonucleotide Synthesis," DNA Abstract (1985), 4:93.
Sanger et al., "DNA Sequencing with Chain-Terminating Inhibitors," PNAS USA (1977), 74:5463-5467.
Smith et al., "The Synthesis of Oligonucleotides Containing an Aliphatic Amino Group at the 5' Terminus: Synthesis of Fluorescent DNA Primers for Use in DNA Sequence Analysis," Nucleic Acids Research (1985), 13:2399-2412.
Smith et al., "Fluorescence Detection in Automated DNA Sequence Analysis," Nature (1986), 321:674-679.
Stryer, "Fluorescence Energy Transfer as a Spectroscopic Ruler," Ann. Rev. Biochemistry (1978), 47:819-846.
Stryer and Haugland, "Energy Transfer: A Spectroscopic Ruler," PNAS USA (1967), 58:719-726.
Theisen et al., "Fluorescent Dye Phosphoramidite Labeling of Oligonucleotides," Tetrahedron Letters (1992), 33:5033-5036.

Affiliated with

Also associated with

Rating

Probes labelled with energy transfer coupled dyes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Probes labelled with energy transfer coupled dyes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Probes labelled with energy transfer coupled dyes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1564666