Method of determining propensity of dissolved oxygen instability

Details Method of determining propensity of dissolved oxygen instability Method of determining propensity of dissolved oxygen instability

1994-05-09

1996-01-30

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 691, C12Q 102

Patent

active

054879809

ABSTRACT:
A process for producing a polypeptide of interest from fermentation of bacterial host cells comprising nucleic acid encoding the polypeptide is provided. In this method, the host cells employed have an inactivated electron transport chain. Further provided is a method for determining if a particular bacterial cell culture has a propensity for dissolved oxygen instability when fermented on a large scale.

REFERENCES:
patent: 4476224 (1984-10-01), Adler
patent: 4762784 (1988-08-01), Keith et al.
patent: 5049493 (1991-09-01), Khosla et al.
patent: 5182195 (1993-01-01), Nakahama et al.
Lin, N. S., et al., (1992), Method 4, 159-168.
Cotter et al., "Cytochrome o (cyoABCDE) and d (cydAB) oxidase gene expression in Escherichia coli is regulated by oxygen, pH, and the fnr gene product" J. Bacteriology 172(11):6333-6338 (1990).
Andersson, D. I., et al., "Redox Regulation of the Genes for Cobinamide Biosynthesis in Salmonella typhimurium", Journal of Bacteriology, 171:6734-6739 (1989).
Anraku et al., "The aerobic respiratory chain of Escherichia coli ", TIBS, 12:262-266 (1987).
Au et al., "Isolation and Characterization of an Escherichia coli Mutant Lacking the Cytochrome o Terminal Oxidase", Journal of Bacteriology, 161(1):123-127 (1985).
Au et al., "Role of Quinones in the Branch of the Escherichia coli Respiratory Chain that Terminates in Cytochrome o", Journal of Bacteriology, 157(1):122-125 (1984).
Brock et al., Biology of Microorganisms, Prentice Hall, N.J. 673 (1988).
Bryan et al., "Aminoglycoside-Resistant Mutants of Pseudomonas aeruginosa Deficient in Cytochrome d, Nitrate Reductase, and Aerobic Transport", Antimicrobial Agents and Chemotherapy, 19(6):958-964 (1981).
Caster et al., "Photochemical Determination of the Oxidases of Bacteria", J. Biol. Chem., 234(6):1587-1592 (1959).
Center for Drugs and Biologies and Center for Devices and Radiological Health FDA, "Guideline on General Principles of Process Validation", May 1987.
Chepuri et al., "Recent studies of the cytochrome o terminal oxidase complex of Escherichia coli", Biochim. Biophysica Acta, 1018:124-127 (1990).
Daldal, Fevzi, "Cytochrome C.sub.2 -Independent Respiratory Growth of Rhodobacter capsulatus", 170(5):2388-2391 (1988).
Dassa et la., "A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA)", Mol. Gen Genet, 229:341-352 (1991).
Degn et al., "Theory of Oscillations of Respiration Rate in Continuous Culture of Klebsiella aerogenes", J. Theoret. Biol., 22:238-248 (1969).
Denis et al., "Oxygen Regulation of Nitrate Transport by Diversion of Electron Flow in Escherichia coli", J. Biological Chem, 265(30):18095-18907 (1990).
Deutch, C. E., "Association of the PutA Protein with the Cell Membrane in Escherichia coli Requires Components of the Aerobic Electron Transport Chain", Abstract K-97, 92nd Gen'l Mtg AM Soc for Microbiology, (1992).
Dikshit et al., "The Bacterial Hemoglobin from Vitreoscilla Can Support the Aerobic Growth of Escherichia coli Lacking Terminal Oxidases", Archives Biochemistry and Biophysics, 293(2):241-245 (1992).
Doelle et al., "Transitional Steady-State Investigations during Aerobic-Anaerobic Transition of Glucose Utilization by Escherichia coli K-12", Eur. J. Biochem., 83(2):479-484 (1978).
Edwards et al., "Photochemical Action Spectra Of Bacterial a-And o-Type Oxidases Using A Dye Laser", FEBS Letters, 128:(2):205-207 (1981).
Fang et al., "Location of Heme Axial Ligands in the Cytochrome d Terminal Oxidase Complex of Escherichia cola Determined by Site-directed Mutagenesis", J. Biol. Chem., 264(4):8026-8032 (1989).
Fu et al., "The requirement of ArcA and Fnr for peak expression of the cyd operon in Escherichia coli under microaerobic conditions", Mol Gen Genet, 226:209-213 (1991).
Green et al., "Identification of Subunit I As the Cytochrome b.sub.558 Component of the Cytochrome d Terminal Oxidase Complex of Escherichia coli", J. Biol. Chem., 259(12):7994-7997 (1984).
Green et al., "Isolation and Characterization of an Escherichia coli Mutant Lacking Cytochrome d Terminal Oxidase", J. Bacteriology, 154(3):1269-1275 (1983).
Green et al., "Isolation and Characterization of an Escherichia Coli Mutant Lacking Cytochrome D.", Fed. Proc., 40: Abstract 1669 (1981).
Green et al., "The Nucleotide Sequence of cyd Locus Encoding the Two Subunits of the Cytohrome d Terminal Oxidase Complex of Escherichia coli", J. Biological Chemistry, 263(26):13138-13143 (1988).
Green et al., "Biochemical and Genetic Characterization of an Escherichia Coli Mutant Lacking Cytochrome d", Fed. Proc. (41):Abstract 3652 (1982).
Georgiou et al., "Identification of the cycC Locus Required for Expression of the Functional Form of the Cytochrome d Terminal Oxidase Complex in Escherichia coli", J. Bacteriology, 169(5):2107-2112 (1987).
Haddock et al., "Electron-Transport Chains of Escherichia coli; Reconstitution of Respiration in a 5-Aminolaevulinic Acid-Requiring Mutant", Eur. J. Biochem., 35:34-45 (1973).
Haltia et al., "Deletion of the gene for subunit III leads to defective assembly of bacterial cytochrome oxidase", The EMBO Journal, 8(12):3571-3579 (1989).
Harrison et al. "The Effect of Growth Conditions on Respiratory Activity and Growth Efficiency in Facultative Anaerobes Grown in Chemostat Culture", Journal of Gen. Microbiology, 68:35-43 (1971).
Harrison et al., "The Influence of Dissolved Oxygen Concentration on the Respiration and Glucose Metabolism of Klebsiella aerogenes during Growth", J. Gen. Microbiology, 46:193-211 (1967).
Hashimoto et al., "Isolation of the Mutants of Escherichia coli that are Defective in Some Rspiratory Enzymes", J. Science of the Hiroshima Univ., Series B, Div. 2, Botany, 15:103-113 (1975).
Hoffman et al., "Respiratory-Chain Characteristics of Mutants of Azotobacter vinelandii Negative to Tetramethyl-p-phenylenediamine Oxidase", Eur. J. Biochem., 100:19-27 (1979).
Hoffman et al., Studies of Photochemical Action Spectra on N,N,N,N'-Tetramethyl-p-phenylenediamine-Oxidase-Negative Mutants of Azotobacter vinelandii, Eur. J. Biochem., 105:177-185 (1980).
Hopkins et al., "Effects of Dissolved Oxygen Shock on the Stability of Recombinant Escherichia coli Containing Plasmid pKN401", Biotechnology and Bioengineering, 29:85-91 (1987).
Iuchi et al., "Requirement for Terminal Cytochromes in Generation of the Aerobic Signal for the arc Regulatory System in Escherichia coli; Study Utilizing Deletions and Iac Fusions of cyo and cyd", J. Bacteriology, 172(10):6020-6025 (1990).
James et al., "The cytochrome oxidases of Bacillus subtilis: mapping of a gene affecting cytochrome as.sub.3 and its replacement by cytochrome o in an muant strain", FEMS Microbiology Letters, 58:277-282 (1989).
Kelly et al., "Cloning and Mutagenesis of Genes Encoding the Cytochrome bd Terminal Oxidase Complex in Azotobacter vinelandii: Mutants Deficient in the Cytochrome d Complex Are Unable To Fix Nitrogen in Air", J. Bacteriology, 172(10):6010-6019 (1990).
Krantz et al., "Immunilogicla Characterization of an Escherichia coli Strain Which Is Lacking Cytochrome d", J. Bacteriology, 156(1):115-121 (1983).
Kranz et al., "Immunological Investigation of the Distribution of Cytochromes Related to the Two Terminal Oxidases of Escherichia coli in Other Gram-Negative Bacteria", J. Bacteriology, 16(2):709-713 (1985).
Kwong et al., "On-Line Assessment of Metabolic Activities Based on Culture Redox Potential and Dissolved Oxygen Profiles During Aerobic Fermentation", Biotechnol. Prog., 8:576-579 (1992).
Laszio et al., "Cytochrome o as a Terminal Oxidase and Receptor for Aerotaxis in Salmonella typhimurium", J. Bacteriology, 159(2):663-667 (1984).
Lemieux et al., "Determination of the Ligands of the Low Spin Heme of the Cytochrome o Ubiquinol Oxidase Complex Using Site-directed Mutagenesis", J. Biological Chemistry, 267(3):2105-2113 (1992).
Li et al., "Effect of the levels of dissolved oxygen on the expression of recombinant proteins in four recombinant Escherichia coli strains", Journal of Industrial Microbiology, 9:1-10 (1992).
Lin et al., "Production of He

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