Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1994-03-11
1997-10-14
Ulm, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
435 691, 536 235, C12N 1512, C07K 1447
Patent
active
056774214
ABSTRACT:
A novel expression cloning method is provided for the detection, identification and purification of target proteins capable of binding at least to a tryosine-phosphorylated domain of a eukaryotic tyrosine kinase using novel peptide probes comprising an amino acid sequence substantially corresponding to a portion of a tyrosine-phosphorylated domain of a tyrosine kinase. The probe has at least one phosphorlated tyrosine residue and may be detectably labeled. Also disclosed is a method for preparing the probe, a method for mapping to a chormosome a gene encoding a protein capable of binding to tyrosine-phosphorylated domains of tyrosine kinases, and a method for purifiying such a protein with the probe. Non-limiting examples of novel proteins/discovered using the above cloning method include GRB-1, GRB-2, GRB-3, GRB-4 and GRB-7, as well as nucleic acid encoding these proteins, and methods for detecting these proteins are also provided.
REFERENCES:
E.Y. Skolnik et al. "Cloning of PI3 Kinase-Associated p85 Utilizing a Novel Method for Expression/Cloning of Target Proteins for Receptor Tyrosine Kinases." Cell. vol. 65, Apr. 5, 1991. pp. 83-90.
Jaime A. Escobedo et al. "cDNA Cloning of a Novel 85 kd Protein That Has SH2 Domains and Regulates Binding of PI3-Kinase to the PDGF .beta.-Receptor." Cell. vol. 65, Apr. 5, 1991. pp. 75-82.
Masayuki Otsu et al. "Characterization of Two 85 kd Proteins That Associate with Receptor Tyrosine Kinases, Middle-T/pp60.sup.c-src Complexes, and P13-Kinases." Cell. vol. 65, Apr. 5, 1991. pp. 91-104.
B. Margolis et al. "The tyrosine phosphorylated carboxyterminus of the EGF receptor is a binding site for GAP and PLC-.tau.." EMBO Journal. vol. 9, No. 13, 1990. pp. 4375-4380.
Pascale F. Macgregor et al. "Direct cloning of leucine zipper proteins: Jun binds cooperatively to the CRE with CRE-BP1." Oncogene. vol. 5, 1990. pp. 451-458.
Axel Ullrich et al. "Signal Transduction by Receptors with Tyrosine Kinase Activity." Cell. vol. 61, 1990. pp. 203-212.
Margolis Benjamin L.
Schlessinger Joseph
Skolnik Edward Y.
New York University
Ulm John
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