Method for producing the HgiAI restriction endonuclease and meth

Details Method for producing the HgiAI restriction endonuclease and meth Method for producing the HgiAI restriction endonuclease and meth

1987-12-17

1991-01-22

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435199, 43525233, 4353201, 536 27, 935 29, 935 73, 935 80, C12N 1552, C12N 922, C12N 121

Patent

active

049870742

ABSTRACT:
The present invention is directed to a method for cloning and producing the HgiAI restriction endonuclease by (1) introducing the restriction endonuclease gene from Herpetosiphon giganteus into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the HgiAI restriction endonuclease, and (3) purifying the HgiAI restriction endonuclease from the fermented host which contains the vector encoding and expressing the HgiAI restriction endonuclease activity.

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