Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-09-29
1986-02-18
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 68, 4351722, 435240, 435948, 436548, 935104, 935110, G01N 3354, C12N 1500, C12N 500, C12R 191
Patent
active
045713812
ABSTRACT:
A continuous hybrid cell line which produces monoclonal antibody directed against the neurotransmitter degrading enzyme, monoamine oxidase B (MAO B), has been developed. The hybrid cell line was established by fusing MAO B primed, differentiated lymphoid cells with myeloma cells. Resulting fused cells were isolated, cloned and characterized as to antibody specificity against antigenic determinants of MAO B. Monoclonal antibody having specificity for MAO B and no cross reactivity with MAO A was selected and implemented in a radioimmunoassay technique for the selective measurement of MAO B concentration independent of its catalytic activity.
REFERENCES:
patent: 4427653 (1984-01-01), Springer
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Abell Creed W.
Denney Richard M.
Tarcza John E.
The University of Texas System & Board of Regents
Wiseman Thomas G.
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