Method of determining the diagnostic significance of the content

Details Method of determining the diagnostic significance of the content Method of determining the diagnostic significance of the content

1983-06-20

1985-05-21

Heyman, John S.

Electrical pulse counters, pulse dividers, or shift registers: c

Applications

Counting animate or inanimate entities

356 39, 382 6, G01N 3348

Patent

active

045190879

ABSTRACT:
The present invention relates to a method for examining the content of a volume of biological sample containing particles to determine the diagnostic significance of that volume of biological sample. A number of aliquots of the sample are distributed and are examined under low power field magnification. The total number of aliquots examined under low power field magnification is a first fraction of the volume of biological sample. The number of particles detected in the first fraction is counted. The number of particles detected is compared to a first known total. In the event the comparison yields the result that the sample contains diagnostically insignificant number of particles, then the method is terminated. Otherwise the method is continued. A second fraction of volume of the biological sample, comprising a plurality of aliquots, is examined under high power field magnification. The number of particles detected under high power magnification is totalled. This is then compared to a second known total. The diagnostic significance of the biological sample is determined by the comparison of the second total number of particles to the number of particles counted in the second fractional volume.

REFERENCES:
patent: 3214574 (1965-10-01), Landsman et al.
patent: 3315229 (1967-04-01), Smithline
patent: 3970841 (1976-07-01), Green
patent: 4021117 (1977-05-01), Gohde et al.
patent: 4061914 (1977-12-01), Green

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