Process and apparatus for growing animal cells

Details Process and apparatus for growing animal cells Process and apparatus for growing animal cells

1980-08-21

1982-08-10

Naff, David M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435285, 435310, 435313, 435315, C12N 500, C12M 114, C12M 106

Patent

active

043439047

ABSTRACT:
Animal cells are grown in a vertically disposed cylindrical vessel containing a stack of parallel spaced-apart discs inclined at least 5.degree. from the horizontal and mounted to a rotatable axial shaft. The vessel is closed by a top plate having a plurality of inlets and a bottom plate with an outlet, and contains an external pumping loop for circulating contents of the vessel from the bottom to the top of the vessel. Growing of the cells is carried out by substantially filling the vessel with a mixture of animal cells and growth medium, allowing the cells to settle on the disc surfaces and then rotating the axial shaft at a speed of at least 5 rpm while continuously circulating the vessel contents from the bottom to the top of the vessel. This process and apparatus provides efficient mixing and ensures a homogeneous system within the vessel.

REFERENCES:
patent: 3407120 (1968-10-01), Weiss et al.
patent: 3905865 (1975-09-01), McAleer et al.
patent: 3933585 (1976-01-01), McAleer et al.
patent: 4208483 (1980-06-01), Lee
patent: 4262090 (1981-04-01), Colby et al.

Affiliated with

Also associated with

Rating

Process and apparatus for growing animal cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process and apparatus for growing animal cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process and apparatus for growing animal cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1531951