Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of detaching cells – digesting tissue or establishing...
Patent
1996-12-13
1998-04-28
Tsang, Cecilia J.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of detaching cells, digesting tissue or establishing...
435379, 435383, 435366, 435353, 435354, C12N 500
Patent
active
057443637
ABSTRACT:
The present invention provides a novel method for the establishment of tumorigenic cell lines from biopsies of human or animal tumors. The method includes preparing a single-cell suspension of a tumor cells by injecting cell culture medium into an isolated tumor or a portion thereof to flush out the tumor cells. The tumor or portion thereof may be in a cell culture maintenance medium. The step of injecting cell culture medium into the isolated tumor or portion thereof involves piercing the tumor or portion thereof with a needle to inject the cell culture medium and flush out the tumor cells. The steps of piercing the tumor or portion thereof with a needle and injecting the cell culture medium to flush out the tumor cells preferably are repeated until the single-cell suspension has a particular cell density. The method also includes establishing a primary tumor cell culture by growing the cells from the single-cell suspension in a cell culture medium.
REFERENCES:
patent: 5273963 (1993-12-01), Moody
patent: 5434132 (1995-07-01), Rozengurt
Jaggi et al., Journal of Basic & Applied Biomedicine, 3(4) : 27-35, 1995.
M. Jaggi et al., "Establishment of Tumorigeric Cell . . . " JBAB-ABS06(mj)12-95 3(4) : 27-35.
X. Fu et al., "A Metastatic Nude-Mouse . . . " Proc. Natl. Acad. Sci, USA, vol. 89, pp. 5645-5649 Jun. 1992.
B. Rae-Ventor et al., "Growth of Human Breast . . . " Cancer Research 40, 95-100 Jan. 1980 pp. 95-98.
P. Giovanella, et al. "Correlation Between Response . . . " Cancer. Oct. 1, 1983, 52 : 1146-1152.
R. Giavazzi et al "Metastatic Behavior . . . " Cancer Research 46, 1928-1933, Apr. 1986.
Peters, L.C. "Preparation of Immunotherapeutic . . . " Cancer Research 39: 1353-1360 (Apr. 1979).
E. Bombardieri, et al., "Somatostatin Receptor Imaging of Small Cell Lung Cancer . . . Scintigraphy," European Journal of Cancer, vol. 31A, No.2, pp.184-188, 1995.
J. Pinski et al., "Somatostatin Analogues and Bombesin/Gastrin . . . in vitro and in vivo," Peptide Analogues in Glioblastomas, vol. , pp.5895-5901, (1994).
P.A. Bunn, Jr., et al., "Effects of Neuropeptide Analogues on . . . Cancer Cell Lines," Cancer Research 54, pp.3602-3610, Jul. 1, 1994.
G. Lilling, et al., "Inhibition of Human Neuroblastoma . . . VIP Antagonist," Journal of Molecular Neuroscience, vol. 5, 1994/1995, pp. 231-239.
H. Reile, et al., "Characterization of High-Affinity Receptors For Bombesin/Gastrin Releasing . . . By Tumor Cells," The Prostate 25:29-38, (1994).
I. Virgolini, M.D., et al., "Vasoactive Intestinal Peptide-Receptor . . . Endocrine Tumors," The New England Journal of Medicine, vol. 331, No. 17, pp.1116-1121, Oct. 27, 1994.
K. Frank-Raue, et al., "Somatostatin Receptor Imaging in Persistent Medullary Thyroid Carcinoma," Clinical Endocrinology (1995) 42, pp. 31-37.
Gabor Halmos, et al., "Characterization of Bombesin/Gastrin-Releasing Peptide . . . Gastric Cancer" Cancer Letters 85 (1994), pp.111-118.
Antal Orosz, et al., "New Short-Chain Analogs of Substance-P . . . Cells in vitro and in vivo," Int. J. Cancer, 60, pp.82-87, (1995).
Karoly Szepeshazi, et al., "Combination of Nitrosamine-Induced . . . Bombesin/GRP Antagonist," Int'l. Journal of Pancreatology, vol. 16, Nos. 2-3, pp.141-149, Oct.-Dec. 1994.
P. Heinz-Erian, et al., "Characterization of a New Group of Substituted Substance P . . . Antagonists," Abstracts of Papers, pp.1455, May 1986.
Tim Mosmann, "Rapid Colorimetric Assay For Cellular Growth and . . . Cytotoxicity Assays," Journal of Immunological Methods, 65, (1983), pp.55-63.
I. Zachary, et al., "Bombesin, Vasopressin, and Endothelin Rapidly Stimulate . . . 3T3 Cells," Proc. Natl. Acad. Sci. USA, vol. 88, pp.4577-4581, Jun. 1991.
I. Gozes, et al., "Vasoactive Intestinal Peptide Potentiates Sexual Behavior . . . Antagonist," Endocrinology, vol. 125, No. 6, pp.2945-2949, 1989. Substance P, A Potent Bombesin . . . in vitro," Proc. Natl. Acad. Sci. USA, vol. 85, pp.1859-1863, Mar. 1988.
M. Jaggi, et al., "New Sensitive and Specific Elisa For Detection . . . Supernatants," Journal of Immunoassay, 15(2), pp. 129-146 (1994).
K. Gulya, et al., "Cyclic Somatostatin Octapeptide Analogues With High . . . Opioid Receptors," Life Sciences, vol. 38, No. 24, pp.2221-2229, 1986.
M. Brown, et al., "Somatostatin: Analogs With Selected Biological Activities," Science, vol. 196, pp.1467-1469, (1977).
J. T. Pelton, et al., "Design and Synthesis of Conformationally Constrained Somatostatin . . . Receptors," J. Med. Chem. 1986, 29, pp.2370-2375.
M. Jaggi, et al., "Establishment of Tumorigenic Cell Lines From Biopsies . . . Adenocarcinomas," Journal of Basic & Applied Biomedicine (1995) 3 (4), pp. 27-35.
Jaggi Manu
Mukherjee Rama
Delacroix-Muirheid C.
National Institute of Immunology
Tsang Cecilia J.
LandOfFree
Method for establishing a tumor-cell line by preparing single-ce does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for establishing a tumor-cell line by preparing single-ce, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for establishing a tumor-cell line by preparing single-ce will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1531364