Immobilization of nucleic acids on solvolyzed nylon supports

Details Immobilization of nucleic acids on solvolyzed nylon supports Immobilization of nucleic acids on solvolyzed nylon supports

1985-09-30

1989-02-21

Warren, Charles F.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

935 78, 435 6, 435803, C07H 2100, C12Q 168

Patent

active

048066311

ABSTRACT:
Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon whose amide groups have been partially solvolyzed. Solvolysis of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by addition of water. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample.

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