Procedure for the detection of allergen-containing house dust

Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-n

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436 8, 436164, 436169, 436178, 436179, 436183, 436513, 436903, G01N 2178

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048064904

ABSTRACT:
The invention relates to a procedure to establish the presence of allergen-containing housedust, as results when or after housedust mites occur, in which samples of dust present in buildings are thoroughly suspended in a aqueous-alcoholic alkali metal hydroxide solution and, with the resulting extract, a color reaction using an aromatic diazo compound is carried out, the color reaction being evaluated with respect to its occurrence as an indication of the pollution of the dust with housedust mite residues. According to a particular embodiment, a paper treated with the aromatic diazo compound (and where appropriate with starch) is used for carrying out the color reaction. A further aspect of the invention involves quantitatively determining the allergological activity resulting from housedust mites in housedust samples.

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